Taxonomic identification report

Identification of candidate species

Flag 1E: Identification not possible (potential unknown species)
No candidate species matched

Taxa of interest (TOI)

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest	Match rank	Match taxon	Match species	Match accession	Match identity	Database coverage
Empoascanara	Did not match any candidate					Database coverage of Taxon of Interest Empoascanara This Taxon of Interest has been independently evaluated against the reference database to determine whether sufficient reference data exists to support identification of this taxon. Insufficient coverage can result in that taxon not be correctly identified as the taxonomic identity of the sample (type-II error). For example, if your sample is from Homo sapiens, but there are no Homo sapiens DNA records in the reference database, the analysis will be unable to detect Homo sapiens as the correct taxonomic identity. In the latter case, the analysis will most likely try to assign the closest relative with existing reference data as the taxonomic identity. Coverage of Empoascanara Coverage of species in genus Empoascanara Coverage of species in genus Empoascanara in country of origin Australia Database coverage of Empoascanara Flag 5.1A: The reference data supports this taxon well Reasoning: The given locus for this taxon is well represented in reference database (>5 entries) 20 records There are 20 sequences in the reference database for Empoascanara at the given locus COI. Global occurrence records for Empoascanara. Note that the occurrence data are not exhaustive, and it is possible for this species to occur in regions not shown on the map. Database coverage of species in genus Empoascanara Flag 5.2C: The reference data offers little support for species in this genus Reasoning: ≤10% of taxon have reference sequence(s) at the given locus / (%) of sequence records were found in the reference database for: Species in the genus Empoascanara At the target locus COI Per-species reference sequence counts Number of GenBank records at locus COI Database coverage of species in genus Empoascanara that occur in country of origin Australia Flag 5.3B: The reference data offers some support for species in this genus, when we evaluate only species that occur in the sample’s country of origin Reasoning: Not all species in genus from the country of origin have reference sequence(s) for this locus / (%) of sequence records were found in the reference database for: Species in the genus Empoascanara At the target locus COI In the sample country of origin Australia Per-species reference sequence counts Number of GenBank records at locus COI

Reference sequence source diversity

This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

No candidate species to report on.

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.